Keywords
microsatellites; DNA; population; genetic diversity;
Pages
pp. 151 -
156

By sequencing ISSR DNA fragments of the Austrian snail (Caucasotachea vindobonensis), cloned into the pAL2-T vector, sequences of microsatellite loci (STR) and their flanking regions were obtained. For the nine identified STR loci with 2, 3 and 4 repeats, the design of primers adapted for fragment analysis was carried out. Subsequent genotyping of 100 individuals of the Austrian snail from various populations of Eastern Europe to estimate the volume allelic diversity of the species based on detected STR loci. All loci yielded unambiguous genotypes. Eight STR loci turn out to be polymorphic and have from 2 to 19 alleles.The data obtained can be used to assess the genetic diversity and phylogenetic analysis of populations of the Austrian snail, listed in the Red Book of the Belgorod region of Russia and the protection lists of Europe.

DOI
10.35885/ruthenica.2024.34(4).1
Разработка первых микросателлитных маркеров для австрийской улитки Caucasotachea vindobonensis (Gastropoda; Pulmonata)
Сычёв А.А.; Юсупова А.Ю.; Снегин Э.А.;